IPLab for Windows - What's New in IPLab 4.0

New Features

• Acquire 6D
• Expanded capability for multi-position and array (multi-well plate) based live cell imaging experiments requiring an automated microscope and X-Y stage.
• Acquire 6D adds control for three shutters plus a filter wheel, AutoFocus, XY stage and volume/filter scans.
• AutoFocus: hardware-based and software-based
• XY Stage Control: visit random positions, display array windows, shortest path sort, Add/Delete positions, Zoom In/Out
• Fast & Slow Z Scan
• Stack Processing
• Add 3rd Shutter control
• Output folder names can be fixed
• Contiguous buffers for Fast Capture
• Display Pseudocolor in Preview

AutoFocus

• Software-based and Hardware-based (external AF device)
• IPLab hardware autofocus scan
• AutoFocus options, with stored settings and access during preview
• AutoFocus - new fields for Resolution, Z-offset, and total number of planes
• Total Distance added to AutoFocus Dialog

Variable time frequency capture

• Pseudocolor images (choose color, wavelength, pseucolor index)
• Preview (Single shot, expose all, histogram, arrange windows)

RGB Color Acquire

• Reset Button for SetColorBalance Values

Image Sequences

• Completed Stamp Overlay/Time on Sequence
• Frames To Sequence Update (Folder options)
• Version Information added to Help menu
• New Script and Help icons removed from main toolbar

Advanced Analysis Toolbar

• Provides simply interface for setting up experiments and making measurements or counts.
• Save the experimental protocol for future use.
• Can be customized for your own applications; scriptable for batch processing.
• Steps include background correction, segmentation, segment modification, setting report and options, measuring segmented objects.

Quantify Multiple Segments (Counting Objects Within Objects)

• Object Mask counts larger objects
• Probe 1 and Probe 2 objects are counted and colocalized inside Object Mask.

Improved Features - IPLab 4.0

Expanded and Improved Camera Control Features

Camera Toolbar

• Live Preview creates a live image preview window and displays the Live Preview controls: exposure time, auto exposure, camera binning, region of interest, and brightness, contrast, and gamma.
• Snapshot grabs the live image preview and creates a new, still image window. Controls: exposure and binning.
• Multidimensional Acquire adds time-lapse, streaming, shutter control, and frame averaging.

Device Select

• Ability to select more devices
• Option to show or hide a device that has been selected
• Choose a display name other than the default name for a device
• Import/Export Device select settings
• Tooltips for Device toolbar / Fkeys

Linked Devices

• Linking devices only shows dialog when scripting
• Select Devices XYZ have a None option so user can unselect a stage device

Linked Windows

• Window size
• ROIs
• Zoom Level
• Current Z&T frame
• Scroll Offset
• Zoom In/Out with toolbar

Support for New Cameras and Automation Devices - IPLab 4.0

Support for New Cameras

• Cooke PCO 1600, 2000, 4000
• Photometrics CoolSNAP HQ2, ES2, EZ, CF2 Firewire
• QImaging Retiga-SRV, Rolera-XR

Support for New Devices

• Ludl Piezo Z-Axis Focus Drive

What's New - Previous Versions

IPLab versions 3.7 through 3.5

Continue Reading

Please continue reading about IPLab:

• Features: A summary list of IPLab's most useful features
  • New IPLab/Win Features : The release notes for IPLab for Windows
• Extensions: Software that adds abilities to IPLab
• Image Acquisition: An entire section about cameras and collecting images
• System Requirements: The minimum computer systems needed to run IPLab